Ice -COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations
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چکیده
منابع مشابه
Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations
Identifying low-abundance mutations within wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. However, utilizing the clinical and diagnostic potential of rare mutations is limited by sensitivity of the molecular techniques employed, especially when the type and position of mutations are unknown. We have developed a novel platf...
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BACKGROUND Low-level mutations in clinical tumor samples often reside below mutation detection limits, thus leading to false negatives that may impact clinical diagnosis and patient management. COLD-PCR (coamplification at lower denaturation temperature PCR) is a technology that magnifies unknown mutations during PCR, thus enabling downstream mutation detection. However, a practical difficulty ...
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Detection of low-level DNA variations in the presence of wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. PCR-based methods to enrich mutations during amplification have limited multiplexing capability, are mostly restricted to known mutations and are prone to polymerase or mis-priming errors. Here, we present Differential S...
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BACKGROUND Multiplex detection of low-level mutant alleles in the presence of wild-type DNA would be useful for several fields of medicine including cancer, pre-natal diagnosis and infectious diseases. COLD-PCR is a recently developed method that enriches low-level mutations during PCR cycling, thus enhancing downstream detection without the need for special reagents or equipment. The approach ...
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Polymerase chain reaction (PCR) is a technique widely used in fungal research. One of its advantages is the ability to amplify very small amounts of DNA, in the picograms range, even in the presence of diverse contaminants. In spite of this, most of the extraction protocols of fungal DNA are designed for the obtention of microgram amounts of very pure DNA, requiring the establishment of relativ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2010
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkq899